dengue virus replication

1996. In this study, we developed a reverse transcription (RT)-real-time PCR assay to quantify positive- and negative-sense RNA of dengue virus type 2 within the cells. Mackenzie JM, Jones MK, Young PR. PMID: 31538308 Competitive suppression of dengue virus replication occurs in chikungunya and dengue co-infected Mexican infants. A slow, efficient and safe nanoplatform of tailored ZnS QD-mycophenolic acid conjugates for in vitro drug delivery against dengue virus 2 genome replication† Ranjeet Dungdung , ‡ a Manikanta Bayal , ‡ b Lathika Valliyott , a Unnikrishnan Unniyampurath , c Swapna S. Nair * b and Rajendra Pilankatta * a Transmission is critically dependent upon the replication of these viruses in both human and mosquito hosts. The causative agent, dengue virus (DENV), is primarily transmitted by the mosquito Aedes aegypti, a species that has proved difficult to control using conventional methods.The discovery that A. aegypti transinfected with the wMel strain of Wolbachia showed limited DENV replication led to trial … Viral replication involves the following steps: The epidemiology of dengue has undergone profound changes in recent years, … Replication of flaviviruses takes place on induced vesicles derived from the ER and requires the viral nonstructural (NS) proteins. A novel in vitro replication system for Dengue virus. Cell Host & Microbes 5:365–375 CrossRef Google Scholar. Monocytes/macrophages are the principal target cells for dengue Intact platelets (squares) or platelets subjected to 3 cycles of freeze-thaw at −80°C (circles) (5 × 10 5 /mL) were incubated with DENV2 (5 × 10 6 virus particles/mL) for 1 hour. DENV and CHIKV were identified by RT-PCR; ELISA was … albopictus).These are the same types of mosquitoes that spread Zika and chikungunya viruses.. Despite the serious socio-economic impact of DENV-associated diseases, antiviral therapy is missing. DENV replicates in the cytoplasm of infected cells and induces a membranous replication organelle, formed by invaginations of the endoplasmic reticulum membrane and designated vesicle packets (VPs). Dengue virus (DENV) is a positive-sense RNA virus transmitted by mosquito vectors (primarily Aedes spp.) Flavonoids against dengue virus type-2 (DENV-2) The Dengue virus (DENV) belongs to the Flavivirus class of the family Flaviviridae. During replication, the virus RNA polymerase (the non-structural 5 protein) synthesizes negative-strand RNA molecules that will further be used as templates for positive-strand RNA, which in … Learn More Dengue Clinical Case Management E -learning National Center for Emerging and Zoonotic Infectious Diseases DENV Replication. Positive-strand RNA viruses are known to rearrange cellular membranes to facilitate viral genome replication. Dengue virus binding and replication by platelets Dengue virus (DENV) infection causes ∼200 million cases of severe flulike illness annually, escalating to life-threatening hemorrhagic fever or shock syndrome in ∼500,000. Using high-resolution mass spectrometry, we have analyzed the temporal metabolic perturbations that occur following dengue virus infection of the midgut tissue. Thus, this study describes a novel mechanism of an IFN-inducible inhibitory factor for DENV and provides the basis for future development of broad-spectrum antivirals against infectious viral diseases, including dengue. Tudor-SN expression was upregulated early after dengue virus infection and was subsequently positively correlated with viral loads in the midgut. Background. Although the replicative life cycle of viruses differs greatly between species and category of virus, there are six basic stages that are essential for viral replication. Although it is well established that dendritic cells and macrophages are primary sites of DENV replication, it remains unclear whether non-hematopoietic cellular compartments serve as virus … Severe clinical outcomes such as dengue hemorrhagic fever and dengue shock syndrome are potentially fatal. Increasing concentration of α-mangostin inhibited virus replication and reduced inflammatory cytokines expression at 24- and 48-h post infection. DENV causes diseases such as dengue fever (DF) and dengue haemorrhagic fever (DHF)/dengue shock syndrome in human that are the most prevalent arthropod‐borne viral diseases in subtropical and … Composition and Three-Dimensional Architecture of the Dengue Virus Replication and Assembly Sites - PubMed. Dengue viruses infect a wide range of human and nonhuman cell types in vitro. Dengue virus (DENV) causes an estimated 390 million infections per year, making dengue the most prevalent mosquito-borne viral infection worldwide [].DENV belongs to the Flaviviridae family and four antigenically distinct virus serotypes designated 1 to 4 (DENV 1–4) have been identified to date. Dengue virus (DENV) is the most common vector-borne viral infection worldwide with approximately 390 million cases and 25,000 reported deaths each year. MicroRNAs (miRNAs) are small non-coding RNA molecules responsible for the regulation of gene expression by repressing mRNA translation or inducing mRNA degradation. This mosquito-borne illness is endemic in most tropical and subtropical countries and has spread significantly over the last decade. Welsch S et al (2009) Composition and three-dimensional architecture of the dengue virus replication and assembly sites. Primarily, the virus will make a full length copy of the complementary, minus strand genome. The maximum non-toxic dose (MNTD) of RC-1 concentration in Vero cells was found to be 150 μM for all of the three incubation periods, 24, 48 and 72 hrs (Figure 5A). Monocytes/macrophages are the principal target cells for dengue A crystal structure of the Dengue virus NS5 protein reveals a novel inter-domain interface essential for protein flexibility and virus replication. Dengue virus: structure, serotypes and mode of transmission Introduction. The bacteria, in that space, compete for the resources and it becomes harder for the dengue virus to sustain its replication. THE DENGUE VIRAL REPLICATION CYCLE. We found that the mutation increased sNS1 production coupled with slightly reduced infectious virus production in vitro and in vivo. Staining of … Zaidi MB (1) (2), Garcia-Cordero J (3), Rivero-Gomez R (1), Corzo-Gomez J (3), González Y Almeida ME (1), Bonilla-Moreno R (3), Bustos-Arriaga J (4), Villegas-Sepulveda N (3), Flores-Romo L (5), Cedillo-Barron L (6). In addition to its membrane altering property, NS4A is a scaffold for the virus replication complex and undergoes oligomerization. Replication Flavivirus cannot replicate in the nucleus because, like most other RNA viruses, it uses the host cell's RNA dependant RNA polymerase to replicate. Each serotype is comprised of multiple distinct genotypes, and include sylvatic genotypes that infect wild non-human primates (NHP). The results presented here show that a small molecule, IU1, of the deubiquitinating enzyme USP14, a component of the host ubiquitin-proteasome protein-degradation system, strongly inhibits Dengue virus replication as well as reduces West Nile virus, Yellow Fever virus, and La Crosse virus replication. Dengue virus translation and replication occur in the endoplasmic reticulum of host cells, which experiences rearrangement and expansion during infection. The bacteria, in that space, compete for the resources and it becomes harder for the dengue virus to sustain its replication. A novel in vitro replication system for Dengue virus. Gastrointestinal bleeding is a hallmark of severe dengue disease and previous reports implicate inflammatory cytokines such as TNF-α as a major player in this event ().However, primate models of dengue infection have shown gastrointestinal tract to be one of the sites of virus replication but … New Delhi: A yet-to-be peer-reviewed study by three laboratories of the government’s Council for Industrial and Scientific Research has found that the plant and root extract of velvetleaf can impact the replication of the Sars-CoV-2 virus, which causes Covid-19, by up to 98 percent in cell cultures.. These mosquitoes typically lay eggs near standing water in containers that hold water, like buckets, bowls, animal dishes, flower pots, and vases. The biogenesis and three-dimensional organization of these membranes and the link between replication and virus assembly sites is not fully clear. 10.1128/JVI.76.24.12925-12933.2002 They are small, enveloped viruses containing a single-strand RNA genome of positive polarity . Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells Kleber Juvenal Silva Farias, 1,2 PaulaRenataLimaMachado,1,2 and Benedito Antônio Lopes da Fonseca 1 DepartmentofInternalMedicine,SchoolofMedicineofRibeir aoPreto,UniversityofS aoPaulo,- … Replication and assembly. Dengue Virus and Rotavirus Differentially Affect Permeability Barrier Functions. Autophagosome formation was also observed under the transmission electron microscope. Dengue Virus Replication and Pathogenesis. 5a, b). aegypti females in vivo . The major objective of this work was to explore the possibility of identifying a small molecule inhibitor of the dengue virus protease and assessing its ability to suppress viral replication in cultured cells. Dengue virus (DENV) causes the most prevalent arthropod-borne viral disease in humans. Replication of Dengue Virus Dengue Virus enters host cells by receptor-mediated endocytosis, which involves binding through the interaction between the surface proteins of virion (E) and cellular receptors on the surface of target cell. 5. The dengue virus (DENV) is an important human arbovirus that belongs to the Flaviviridae. Shortly after a virus infects a living cell, it taps into the host's replication machinery to make more copies of itself. Original Article CRISPR-Cas13a Cleavage of Dengue Virus NS3 Gene Efficiently Inhibits Viral Replication Hao Li, 1Shan Wang,2 Xue Dong, 1Qiao Li, Min Li,3 Junfeng Li, Yan Guo, Xia Jin,3 Yusen Zhou,1 Hongbin Song,2 and Zhihua Kou1 1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China; 2Chinese PLA Center for Disease Control Our results support the potential use of α-mangostin as anti-antiviral and anti-inflammatory therapies in the treatment of dengue. Dengue viruses are members of the family Flaviviridae genus Flavivirus. Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication Salomé Cabrera-Romo , Benito Recio-Tótoro , Ana C. Alcalá , Humberto Lanz , Rosa María del Ángel , Victor Sánchez-Cordero , Ángel … Dengue virus (DENV), is a single stranded positive RNA virus encodes 10,173 nucleotides which are translated to form polyprotein [], belonging to the genus Flavivirus and family Flaviviridae.DENV is arbovirus (arthropod-borne) and the vector responsible for transmission of infection is mosquitoes of Aedes genus, more specifically A. aegypti and A. albopictus []. Dengue, caused by dengue virus (DENV) infection, is a public health problem worldwide. In the present study, we observed an increased level of COX-2 in patients with dengue … Mutations of NS4A that affect interaction with NS4B abolished or severely reduced virus replication indicating the importance of NS4A and its … The researchers also demonstrated that NS1 is required for the generation of the membranous dengue virus replication organelle in infected cells. This function does not require RNA replication and is independent from NS1's interaction with NS4A-2K-4B. DENV-1, DENV-2, DENV-3 and DENV-4 are its 4 genotypes. Viral replication involves the following steps: Further studies of the dengue virus life cycle revealed another piece of the virus… DENV suppresses the interferon response, replicates, and causes disease in humans but not wild-type mice. Interestingly, since heat shock treatment at 6 h post-infection also increased viral yields, it is likely that HS also modulates positively dengue virus replication. We have developed a dengue virus replicon system that can be used to discriminate between translation and RNA replication. Dengue virus replication in human hepatoma cells activates NF-kappaB which in turn induces apoptotic cell death. A quinoline compound inhibits the replication of dengue virus serotypes 1-4 in Vero cells. The trial was reported to have used about five million mosquito eggs that were infected with Wolbachia. We screened a siRNA library targeting human tyrosine kinases in Huh-7 cells and identified c-terminal Src kinase (Csk) as one of the kinases involved in dengue virus replication… Blood samples were collected from 24 patients, aged 2 months to 82 years, during a CHIKV outbreak in Mexico. While there are several promising v … Therefore, finding an efficient antiviral agent against this virus is crucial. Dengue virus (DENV) is a mosquito-borne pathogen for which no vaccine or specific therapeutic is available. J Virol 2002,76(24):12925-12933. While dengue virus is thought to replicate in mononuclear phagocytic cells in vivo, attempts to detect it in peripheral blood mononuclear cells (PBMC) by virus isolation or antigen detection have had variable and generally low rates. Virol J 5: 51. Mosquito-borne viruses, such as Dengue virus (DENV), have become global threats to human health in recent years. However, no antiviral drugs have been approved to treat DENV induced diseases, and the safe and effective vaccines are still under development. Although DENV pathogenesis has not yet been fully elucidated, the in Effect of high doses of vitamin D supplementation on dengue virus replication, Toll-like receptor expression, and cytokine profiles on dendritic cells | SpringerLink Inhibition of dengue virus replication by novel inhibitors of RNA-dependent RNA polymerase and protease activities. Dengue Virus Reporter Replicon is a Valuable Tool for Antiviral Drug Discovery and Analysis of Virus Replication Mechanisms. Flaviviruses: Dengue virus, Yellow fever virus, West Nile virus, Zika virus. 2008. Dengue viruses (DENV) infect 50 to 100 million people worldwide per year, of which 500,000 develop severe life-threatening disease. Dengue virus; microRNA; 3’ untranslated region; virus replication Dengue is considered one of the most important diseases transmitted by mosquitos, its incidence has increased at an alarming rate and it has become a public health problem over the last fifty years ( Bhatt et al. Marianneau P(1), Cardona A, Edelman L, Deubel V, Desprès P. Author information: (1)Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, Paris, France. Dengue Virus Medicine & … These viruses are endemic in tropical and subtropical regions of the world placing 2.5 billion people at risk of infection. DV2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral replication … MH488959 The role of COX-2 in dengue virus (DENV) replication remains unclear. Dengue viruses infect a wide range of human and nonhuman cell types in vitro. 69. of Virol. Although the initial rearrangement is independent of the unfolded protein response (UPR), dengue virus alters the UPR in order to cope with endoplasmic reticulum stress throughout infection. @article{osti_21140977, title = {Autophagic machinery activated by dengue virus enhances virus replication}, author = {Lee, Y -R and Lei, H -Y and Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan and Liu, M -T and Wang, J -R and Chen, S -H and Jiang-Shieh, Y -F and Lin, Y -S and Yeh, T -M and Liu, C -C and Liu, H.-S.}, … Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Although miRNAs possess antiviral activity against many … Background: Dengue infection ranks as one of the most significant viral diseases of the globe. Dengue virus (DENV) is a single-stranded positive sense RNA virus and a member of the genus Flavivirus within the family Flaviviridae. A novel coding-region RNA element modulates infectious dengue virus particle production in both mammalian and mosquito cells and regulates viral replication in Aedes aegypti mosquitoes. Dengue is the most important cause of mosquito-borne virus diseases in tropical and subtropical regions in the world. Dengue virus type 2 infection of the ICR suckling mice causes physiopathological changes. Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by Aedes aegypti mosquitoes. Abstract. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Intriguingly, replication of several other viruses, such as hepatitis C virus, Kunjin virus, and Chikungunya virus, was also limited by RyDEN expression. Co-circulation of dengue virus (DENV) and chikungunya virus (CHIKV) is increasing worldwide but information on the viral dynamics and immune response to DENV-CHIKV co-infection, particularly in young infants, is scant. The nonstructural protein 1 (NS1) of the dengue virus interacts with another viral protein called NS4A-2K-4B to enable viral replication, according to a … The agent is a virus with single-stranded RNA, classified into four distinct dengue virus (DENV) serotypes: DENV-1, DENV-2, DENV-3, and DENV-4. We cloned, expressed and purified recombinant dengue virus type 2 protease. ; Dengue virus is transmitted between people by female mosquitoes Ades aegypti and Ades albopidus, which are found throughout the tropical and sub-tropical regions. Platelet-dependent replication of the Dengue virus genome. Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein. Role of Botanical Drugs in Controlling Dengue Virus Disease By Dr. Ravindra B. Malabadi Antiviral activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against dengue virus type 2 (GenBank accession no. Here we investigated how the integrated stress response (ISR), which promotes translational arrest by eIF2α phosphorylation (p–eIF2α), regulates Flavivirus replication. Gamarnik's team published those findings in the June 2005 issue of the Journal of Virology. Groat-Carmona AM, Orozco S, Friebe P, Payne A, Kramer L, and Harris E. 2012. PNAS 108(19): 8003-8008. The trial was reported to have used about five million mosquito eggs that were infected with Wolbachia. A better understanding of the flavivirus replication cycle is likely to offer new opportunities for the design of antiviral therapies to treat severe conditions provoked by these viruses, but it should … Viruses from the Flavivirus family are the causative agents of dengue fever, Zika, Japanese encephalitis, West Nile encephalitis or Yellow fever and constitute major or emerging public health problems. Velvetleaf extract shows to inhibit replication of Sars-CoV-2 virus in lab : CSIR Study Mon, Jun 14 2021 Pakistan: Five killed, six injured as windstorm lashes … influenced replication of dengue virus and disease outcome in cell lines, mosquitoes, and type 1 interferon (IFN)–deficient AG129 mice. This also resulted in a significant increase of dengue viral copy number and the amounts of viral NS5 and NS3 proteins in host cells (Fig. The DENV genome is approximately 11,000 nt, consisting of a 5′ untranslated region (UTR), an open reading frame (ORF) encoding a polyprotein, C-prM-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5, and a 3′ UTR. This is the primary site of infection and replication, preceding systemic viral dissemination and transmission. Initiation of RNA synthesis at the 3′-end of exogenous viral RNA templates requires 5′- and 3′ … Dengue viruses are spread to people through the bites of infected Aedes species mosquitoes (Ae. During viral replication, DENV is taken up by antigen -presenting cells (APC) of the skin known as dendritic cells (Langerhans cells). Using this system, we analyzed the functional role of well-defined RNA elements present at the 3'UTR of dengue virus in mammalian and mosquito cells. We have previously reported the anti-DENV activity of two flavonoids, nam As viruses are obligate intracellular pathogens they cannot replicate without the machinery and metabolism of a host cell. The elevated sNS1 production by T164S mutant virus induced higher Currently, there is no vaccine or effective antiviral agent against DENV. Dengue is one of the most widespread mosquito-borne diseases in the world. Ludert JE, Mosso C, Ceballos-Olvera I, del Angel RM. 80(20): 11418-11431. Introduction. The nonstructural protein 1 (NS1) of the dengue virus interacts with another viral protein called NS4A-2K-4B to enable viral replication, according to a … Promotion of the viral replication by PKC blockage was also confirmed using siRNA-mediated silencing of PKCα. Adelman ZN, Sanchez-Vargas I, Travanty EA, Carlson JO, Beaty BJ, Blair CD, Olson KE: RNA Silencing of Dengue Virus Type 2 Replication in Transformed C6/36 Mosquito Cells Transcribing an Inverted-Repeat RNA Derived from the Virus Genome. We and others have demonstrated that dengue virus infection of various human cell lines induces autophagy, which further promotes virus replication[25, 33, 36].In order to establish the role of DV2 infection in the induction of autophagy as well as its roles in DV replication and DV-related … Attachment: Viral proteins on the capsid or phospholipid envelope interact with Dengue virus (DENV) infection and replication induces oxidative stress, which further contributes to the progression and pathogenesis of the DENV infection. Successful viral replication is … A recent study has found that plant and root extracts of velvetleaf can prevent the replication of the SARS-CoV-2 virus that causes Covid-19 by up to 98% in cell cultures. Dengue virus infection in a cultured cell is revealed by the magenta color, showing that the virus has localized to the endoplasmic reticulum of the host cell. Author summary The Aedes aegypti mosquito transmits arboviruses that cause dengue, Zika, chikungunya and yellow fever. aegypti or Ae. Infection with any of the four DENV serotypes can lead to a broad spectrum of clinical … They are small, enveloped viruses containing a single-strand RNA genome of positive polarity [ 2 ]. Dengue, the most prevalent arthropod-borne viral disease, is caused by the dengue virus (DENV), a member of the Flaviviridae family, and is a considerable public health threat in over 100 countries, with 2.5 billion people living in high-risk areas. Introduction. Currently, there is no specific vaccine or antiviral therapy for prevention or treatment. The host’s innate and adaptive immune responses play an essential role in determining the natural history … Human autopsies revealed staining for DENV structural proteins and negative-sense viral RNA, indicative of virus replication, in M(s in LNs, spleen, lung, and liver and monocytes in clotted blood from patients with lethal dengue disease (38,39). Beesetti H, Tyagi P, Medapi B, Krishna VS, Sriram D, Khanna N, Swaminathan S. Antivir Ther, 23(5):385-394, 01 Jan 2018 Cited by: 0 articles | PMID: 29583121 aegypti derived cell line Aag2 and in the midgut of Ae. (2019) The nonstructural protein 1 (NS1) of the dengue virus interacts with another viral protein called NS4A-2K-4B to enable viral replication, according to a study published May 9 in the open-access journal PLOS Pathogens by Ralf Bartenschlager of the University of Heidelberg, and colleagues. 1 Luteolin Restricts Dengue Virus Replication Through Inhibition of the Proprotein Convertase Furin Minhua Penga,b, Satoru Watanabeb, Kitti Wing Ki Chanb, Qiuyan Hea, Ya Zhaoa,c, Zhongde Zhangd, Xiaoping Lai a, Dahai Luoe, Subhash G. Vasudevanb,* and Geng Li,* aDepartment of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, 1. Although Aedes mosquitoes transmit DENV when probing for blood in the skin, no information exists on DENV infection and immune response in the dermis, where the blood vessels are found. The presence of sub-neutralizing concentrations of antibodies that are cross-reactive between types, elicited by a primary DENV infection, is thought to result in … DENV replicates in the cytoplasm of infected cells and induces a membranous replication organelle, formed by invaginations of the endoplasmic reticulum membrane and designated vesicle packets (VPs). double-stranded RNA (an essential part of the dengue replication DENV is a mosquito-borne single-stranded RNA virus that complex) with LC3, we identified autophagic vacuoles as a site for es belongs to the genus Flavivirus (family Flaviviridae), a genus that at least a part of the dengue virus replication … Together they form a unique fingerprint. The mechanisms by which Flaviviruses use non-canonical translation to support their replication in host cells are largely unknown. Initiation of RNA synthesis at the 3′-end of exogenous viral RNA templates requires 5′- and 3′-terminal complementary sequence motifs of the viral … Background: Dengue infection ranks as one of the most significant viral diseases of the globe. Dengue viruses are members of the family Flaviviridae genus Flavivirus. The inhibitory potential of RC-1 against dengue virus replication in Vero cells. NF90 Binds the Dengue Virus RNA 39 Terminus and Is a Positive Regulator of Dengue Virus Replication Rau´l C. Gomila.¤, Glover W. Martin III , Lee Gehrke* Division of Health Sciences and Technology and Department of Microbiology and Molecular Genetics, Harvard … Introduction. These eggs were put in water buckets in the city every two weeks. The extract that is used by Ayurvedic doctors to treat fever, especially dengue, was found to work like several other antivirals. standing virus spread and disease pathogenesis. ; Structure of Dengue virus: Habitat expansion of Aedes, mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world’s population at risk for DENV infection.After a bloodmeal from a DENV-infected host, the virus … consisting of four, often co-circulating, serotypes in the genus flavivirus. Journ. Currently, there is no specific vaccine or antiviral therapy for prevention or treatment. Its genome is a single positive-stranded RNA-genome and causes various human illnesses like dengue and dengue hemorrhagic fever (DHF) & (DF). Nonstructural protein 1 (NS1) of DENV is a multifunctional protein. Flavivirus is a genus of positive-strand RNA viruses in the family Flaviviridae.The genus includes the West Nile virus, dengue virus, tick-borne encephalitis virus, yellow fever virus, Zika virus and several other viruses which may cause encephalitis, as well as insect-specific flaviviruses (ISFs) such as cell fusing agent virus (CFAV), Palm Creek virus (PCV), and Parramatta River virus (PaRV).

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